ACCIÓN INHIBITORIA DE PROBIÓTICOS SOBRE EL CRECIMIENTO AXÉNICO IN VITRO DEEntamoeba histolytica
Abstract
Entamoeba histolytica es el protozoario parásito cosmopolita causante de la amibiasis y sus múltiples manifestaciones clínicas afectando con mayor frecuencia a los países en vías de desarrollo en donde existe hacinamiento y condiciones sanitarias deficientes e inadecuadas; en México representa un problema de salud
pública por su frecuencia, morbilidad, mortalidad y fácil dispersión; para el control de la amibiasis y sus manifestaciones clínicas, se administra metronidazol como droga de elección, sin embargo este fármaco produce efectos secundarios indeseables. Reportes recientes indican que cultivos in vitro de E. histolytica han desarrollado resistencia a este fármaco. En este trabajo evaluamos el efecto de 1, 10, 20 y 100 mg/mL de liofilizados de medios condicionados con probióticos (Lactobacillus acidophilus, Lactobacillus casei y Lactococcus lactis) sobre el crecimiento axénico in vitro de E. histolytica. Las concentraciones ensayadas mostraron diferencia estadística significativa (ANOVA p=0.05) como inhibidores del crecimiento in vitro de Entamoeba histolytica HM1-IMSS. Estos resultados abren la posibilidad de contar con una alternativa nutricional para la prevención y tratamiento de la amibiasis sin presentar efectos secundarios indeseables.
Palabras claves: Entamoeba histolytica, Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, cultivo axénico, probióticos.
Entamoeba histolytica, Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, axenic culture, probiotics.
Downloads
References
-World Healt Organization. 1997. Amoebiasis-an expert consultation. Weekly Epidemiological Rec. Apr.4;
(14):97-99.
-Lynch, E.C., M.I. Rosenberg and C. Gitler. 1982. An ion chanel forming protein produced by E. histolytica. EMBO. J. 1:801-804
-Campos, G.E. 1996. Incremento de la síntesis de quitina en quistes de Entamoeba histolytica por efecto de Mg2+, Mn2+, y Co 2+. Tesis de Postgrado Nivel Maestría de la Facultad de Ciencias Biológicas, UANL.
-Legator, M.S., T.H. Connor and M. Stoeckel. 1975. Detection of mutagenic activity of metronidazole and niridazole in body fluids of human and mice. Science. 188:1118-1119.
-Samarawickream, N.A., D.M. Brown, J.A. Upcroft, N. Thammapalerd and P. Upcroft. 1997. Involvement of superoxide dismutase and pyruvate: ferredoxin oxidoreductase in mechanisms of metronidazole resistance inEntamoeba histolytica. J. Antimicrob. Hemother. 40:833-840.
-Fariborz, M.G., D. Najaf, Y. Kamyar and S. Afshin. 2003. Efficacy of Saccharomyces boulardii with antibiotics in acute amoebiasis. World J. Gastroenterol. Aug; 9 (8):1832-1833.
-Mombelli, B. and M. Gismondo. 2000. The use of probiotics in medical practice. Int. Antimicrob. Agents.16 (4) 531-536.
-Mc Farland, L.V. 2000. Benefical microbes. Health or hazard?. Eur. Gastroenterol Hepatol, 12 (10) 1069- 1071.
-Penna, F.J. 1989. Diarrea y Probióticos. Simposio sobre utilidad de los probióticos en el manejo de las diarreas. Revista de enfermedades infecciosas en pediatría. Vol X1, número 6, p 182.
-Saxelin, M. 1997. Lactobacillus gg: A human probiotic strain with thorough clinical documentation. Food Rev. Int. 13(2): 293-313.
-Tannock, G.W. 1997. Properties of lactic-acid bacteria: Plenty of scope for fundamental research. TBITECH. 15:270-274.
-Salminen, S., M. Deighton and S. Gorbach. 1993. Lactic acid bacteria in health and disease. In: Lactic acid bacteria (S. Salminen and von Wright, eds.), Marcel Decker, Inc. New York. Pp.199-225.
-Saloff-Coste, C.J. 1997. Beneficios de las leches fermentadas y de los probióticos sobre la salud: una revisión, Danone World Newsletter. 15: 1-11.
-González, S., G. Ibarracin, R. Locascio, M. Pesce, M. Male, M.C. Apella, R. Pesce, A. Holgado and G. Oliver. 1990. Prevention of infantile diarrhoea by fermented milk. microbiologie aliments nutrition. 8 (4) 349- 354.
-Salminen, S., et al., Op. cit.
-Portier, A., N.P. Boyaka, F. Bougoudogo, M. Dubarry, I. Huneau, D. Tomé, A. Dodin and M. Coste. 1993. Fermented milks and increased antibody responses against cholera in mice. Int J. Immunother. 9:217-224.
-Reid, G. 2000. Probiotics in the treatment of diarrheal diseases. Curr. Infect. Dis. Res. 2 (1):78.
-Link-Amster, H., F. Rochat, K.Y. Saudan, O. Mignot and J.M. Aeschlimann. 1994. Modulation of a specific humoral immune response and changes in intestial flora mediated through fermented milk intake. Fems. Immun. Med. Microbiol.10:55-64.
-Robinson, R.K. and A. Samona. 1992. Healt aspects of bifidus products: a review. Int. J. Food. Sci. Nutr, 43:175-180.
-Perez, P.F., J. Minnard, M. Rouvet, C.H. Knabenhans, D. Brassart, G.L. De Antoni and E.J. Shiffrin. 2001. Inhibition of Giardia intestinalis by acellular factors from Lactobacilli: an in vitro study. Appl. Environ. Microbiol. 67:5037-5042.
-De Man, J.C., M. Rogosa and T. Shapre. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135.
- Diamond, L.S. 1968. Techniques of axenic cultivation of Entamoeba histolytica Schaudinn. 1903 and E. histolytica-like amebae. J. Parasitol 54:1047-1056
-Saíd-Fernández, S., J. Vargas-Villegas, J. Castro-Garza, B.D. Mata-Cárdenas, L. Navarro- Marmolejo, G. Lozano-Garza and H. Martínez-Rodríguez. 1988. PEHPS medium: an alternative for axenic cultivation ofE.histolytica and E. invadens. Trans.R.Soc.Trop Med. Hyg 82:249-253.
-Mata-Cárdenas, B.D., M. R. Morales-Vallarta, J. Vargas-Villegas and S. Saíd-Fernández. 1996. PACSR: A Serum Repleacement for axenic cultivation of Entamoeba histolytica. Trans. Roys. Soc. Trop. Med. Hyg. 90:586.
-De Waard, R., J. Garssen, G. Bokken and J.G. Vos. 2002. Antagonistic activity of Lactobacillus casei strainshirota against gastrointestinal Listeria monocitógenes infection in rats. Int. J. Food Microbiol. 73:93-100.
-Glück, U. and G. Jan-Olaf. 2003. Ingested probiotics reduce nasal colonization with pathogenic bacteria (Staphylococcus aureus, Streptococcus pneumoniae, and Beta-hemolytic streptococci). Am. J. Clinical Nutrition. 77:517-520.
-Sgouras, D., P. Maragkoudakis, K. Petraki, B. Martínez-González, E. Michopoulos, G. Kalantzopoulos, E. Tsakalidou and A. Mentis. 2004. In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota. Appl. Environ Microbiol. 70 :518-526.
-Fariborz, M.G., et al, Op. Cit.
-Perez, P.F., et al, Op. Cit.
-Idem.
-Samarawickream, N.A., et al, Op. Cit.
Downloads
Published
How to Cite
Issue
Section
License
The rights of the work belong to the author or authors, however, by sending it for publication in the Public Health and Nutrition Magazine of the Faculty of Public Health and Nutrition of the Autonomous University of Nuevo León, they grant the right for its first publication in between electronic, and possibly, in print to the Public Health and Nutrition Magazine. The license used is the Creative Commons attribution, which allows third parties to use what is published whenever the authorship of the work is mentioned and the first publication that is in the Public Health and Nutrition Magazine. Likewise, the author or authors will take into account that it will not be allowed to send the publication to any other journal, regardless of the format. The authors will be able to make other independent and additional contractual agreements for the non-exclusive distribution of the version of the article published in the Public Health and Nutrition Magazine (e.g., institutional repository or publication in a book) provided they clearly state that The work was published for the first time in the Public Health Magazine, Magazine of the Faculty of Public Health and Nutrition of the Autonomous University of Nuevo León.
