VIABILIDAD DE QUISTES DE G. LAMBLIA Y OOQUISTES DE C. PARVUMEN EL TRATAMIENTO DE AGUAS RESIDUALES CONVENCIONAL
Abstract
Los quistes de Giardia lamblia y ooquistes de Cryptosporidium parvum causan enfermedades intestinales en animales y seres humanos a través del consumo de agua y alimentos contaminados. Los quistes y ooquistes son resistentes a los sistemas de cloración convencional. La dosis infecciosa mínima es de aproximadamente 10 quistes/ooquistes ingeridos. Para la cuantificación de los quistes y ooquistes se utilizó el método ICR. La determinación de la viabilidad se realizó mediante tinción vital y contraste de fases. Después del proceso de tratamiento en la PTAR los quistes de Giardia lamblia se encuentran viables en un 39% y los ooquistes deCryptosporidium parvum en el 71%. La concentración de quistes de Giardia lamblia y ooquistes de Cryptosporidiumparvum aumenta en otoño. Se observó una diferencia significativa (p <0,05), en la incidencia de quistes de Giardialamblia durante la investigación.
Abstract
Giardia lamblia cysts and Cryptosporidium parvum oocyst come to cause intestinal diseases through the consumption of contaminated food and water by humans and animals. Cysts and oocysts are resistant to normal chlorination systems. The minimum infectious doses are approximately 10 cysts / oocysts ingested. The ICR method was used for quantification of cysts and oocysts. The determination of viability was performed by vital staining.Giardia lamblia cysts were found in greater numbers than Cryptosporidium parvum oocysts. Giardia lamblia cysts were viable at 39% after the treatment process and Cryptosporidium parvum oocysts at 71%. The incidence ofGiardia lamblia and Cryptosporidium parvum is higher in autumn. During the investigation the Giardia lamblia cysts incidence were significantly affected (p <0.05).
Palabras clave: Giardia lamblia, Cryptosporidium parvum, agua, alimentos, contaminación
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References
Olson M. 2002. Cryptosporidium and Giardia: Emerging zoonoses. Veterinary 33(6): 24-27
Mahin T. and O.Pancormo. 1999. Waterborne Pathogens. Water Environ. Technol. 11 (4):51-55.
Park C. and P. Huck. 2003. A Conceptual Model for Cryptosporidium Transport in Watersheds. Water Qual. Res. 38(1): 77-113
Ochiai Y., C. Takada and M. Hosaka. 2005 Detection and Discrimination of C. parvum and C. hominis in Water Samples by Immunomagnetic Separation-PCR. Appl. Environ. Microbiol. 71(2):898-903.
Caccio S., M. Giacomo, F. Aulicino and E. Pozio. 2003. Giardia Cysts in Wastewater Treatment Plants in Italy. Appl. Environ. Microbiol. 69 (6): 3393-3398
Mahin T. and O. Pancormo.,Op.cit.
Ochiai Y., et al, Op. Cit.
Soto B. M., C. Chaidez, P. Gortáres, W. Rubio, C. Martínez, P. Meza y N. Castro. 2004. Incidencia deCryptosporidium y Giardia en Agua de Irrigación del Valle de Culiacán, Sinaloa.. Memoria en extenso. Trabajo No. 13. XIV Congreso Nacional. Federación Mexicana de Ingeniería Sanitaria y Ciencias Ambientales.
Shaber N.A., T.R. Slifko and M. Wanielista. 2008. Occurrence of Cryptosporidium, Giardia, and Metals in Florida Stormwater Ponds and Assessment as Alternative Water Supplies for Irrigation. http://www.stormwater.ucf.edu/conferences/9thstormwatercd/documents/OccurrenceofCrypto.pdf
Norma Oficial Mexicana NOM-003-ECOL-1997 Que establece los límites máximos permisibles de contaminantes para las aguas residuales tratadas que se reúsen en servicios al público. (Publicada en el D.O.F. de fecha 21 de septiembre de 1998)
Waterborne™, Inc. 2007. A100FLK. Aqua-Glo G/C Direct Comprehensive Kit Fluorescein-labeled Monoclonal Antibody Reagent for Simultaneous Direct Immunofluorescence Detection of Giardia Cysts and CryptosporidiumOocysts in Water Samples Aqua-Glo G/C is EPA - approved for use in Methods 1622 and 1623.(Ver:http://www.waterborneinc.com/documents/A100FLK.pdf
García A., W. Yanko, G. Batzer and G. Widmer. 2002 Giardia Cysts in Tertiary- Treated Wastewater Effluents: Are They Infective? Water Environ Res. 74(6):541-544.
Idem.
Díaz M., E. Leyva, V. Mata y H. González. 2003. Incidencia y Viabilidad de Cryptosporidium parvum en el agua potable de Cd. Obregón, Sonora, México. Int. Contam. Ambient. 19 (2): 67-72
OOMAPASC. 2003. Plantas de Tratamiento de Aguas Residuales.
EPA. 1996. ICR Microbial Laboratory Manual. ICR Protozoan method for detecting Giardia cyst and oocyst. http://www.epa.gov/nerlcwww/icrmicro.pdf
Waterborne™, Inc. Op.cit.
EPA. 2005. Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. (Ver: http://www.epa.gov/nerlcwww/1623de05.pdf ).
EPA. 1996. Op. Cit.
Chaidez C. 2004. Incidencia de protozoarios en agua de uso agrícola y su relación con la presencia de Clostridium perfringens. http://www.ciad.edu.mx/salima/display1.asp?func=display&resid=289&tree=565
Caccio S., et al, Op cit.
Soto B. M., et al, Op cit.
Solarte Y., M. Peña y C. Madera. 2006. Transmisión de protozoarios patógenos a través del agua para consumo humano. Colomb. Méd. 37 (1): 74-82.
Carmena D., X. Aguinagalde, C. Zigorraga, J.C Fernández-Crespo and A. Ocio . 2006. Presence of Giardia cysts and Cryptosporidium oocysts in drinking water supplies in northern Spain. Appl. Microbiol. 102(3): 619-629.
Piédrola G. 2000. Medicina preventiva y salud pública. En: Aguas residuales. Editorial Mason S.A. Barcelona, España. pp. 327
Tsuchihashi R., J. Loge and L. Darby. 2003. Detection of Cryptosporidium parvum in Secondary Effluents Using a Most Probable Number-Polymerase Chain Reaction Assay. Water Environ Res. 75 (4): 292-299
Ottoson J., A. Hansen, T. Westrell, K. Johansen, H. Norder and A. Stenström . 2006. Removal of Noro and Enteroviruses, Giardia Cysts, Cryptosporidium Oocysts and Fecal Indicators at four Secondary Wastewater Treatment Plants in Sweden. Water Environ. Res. 8: 828-34
Solarte Y., et al, Op. cit.
Grimason, A. M., H. V Smith, J. F. W Parker, Z Bukhari, A. T.; Campbell and L. J. Robertson, 1994. Application of DAPI and immunofluorescence for enhanced identification of Cryptosporidium spp oocysts in water samples. Water Res. 28(3): 733-736.
Alarcón M.A., Beltrán M., Cárdenas M.L., Campos M.C. 2005. Recuento y determinación de viabilidad de Giardiaspp. y aguas potables y residuales en la cuenca alta del Río Bogotá. Biomédica. 25(3): 353-65
Botero L., I. Arnedo, M. Bracho y O. Díaz 2006. Determinación de la presencia de parásitos y bacteriófagos en un sistema de tratamiento de aguas residuales (Ver:http://www.bvsde.paho.org/bvsaidis/uruguay30/VE04179_Botero.pdf)
García A., et al, Op. Cit
Robertson L.J. and B.K. Gjerde 2006. Fate of Cryptosporidium Oocysts and Giardia Cysts in the Norwegian Aquatic Environment over winter. Microbial Ecol. 52: 597–602.
Solarte Y., et al, Op. cit.
Díaz M., et al, Op.cit.
Ochiai Y., et al, Op. Cit.
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